Using the Griess colorimetric nitrite assay for measuring aliphatic β-nitroalcohols

Aliphatic beta-nitroalcohols for therapeutic corneoscleral cross-linking: chemical mechanisms and higher order nitroalcohols.

PURPOSE The recent tissue cross-linking studies indicate that aliphatic beta-nitroalcohols (BNAs) may be useful as pharmacologic corneoscleral cross-linking agents. The present study was performed to identify the specific chemistry involved under physiologic conditions, with the intent of identifying more effective agents. METHODS The mechanism of chemical cross-linking at pH 7.4 and 37 degre...

Aliphatic b-Nitroalcohols for Therapeutic Corneoscleral Cross-Linking: Chemical Stability Studies Using H-NMR Spectroscopy

Recent studies suggest that aliphatic b-nitro alcohols may represent a useful class of compounds for use as in vivo therapeutic corneoscleral cross-linking agents with higher order nitroalcohols (HONAs) showing enhanced efficacy over the mono-nitroalcohols. The current study was undertaken in order to evaluate the chemical stability of these compounds during storage conditions. Two mono-nitroal...

Colorimetric Assay for Antifungal Susceptibility

A colorimetric assay for mucous glycoproteins using Alcian Blue [proceedings].

The present paper describes a simple, sensitive technique for measurement of respiratory mucous glycoproteins that involves their precipitation with the cationic dye Alcian Blue. The assay was adapted from that described by Whiteman (1973) for measurement of urinary glycosaminoglycans. This method is useful for measurement of mucins secreted in response to stimulating agents in isolated airway ...

A NEW CYTOCOLORIMETRIC ASSAY USING PHOSPHATAS E ACTIVI T Y FOR MEASURING CELLULAR FUNCTIONS

In this study cellular phosphatase activity, referred to as enzyme developed color (EDC) assay, was used for an in vitro assessment of cell proliferation and cytotoxicity. Optimum conditions were established and found to be 10 x103 cells / well at 37°C and 60 min incubation (for developing color). Under the same conditions a direct correlation between optical density (00) and cell number wa...